Skip to content

Yeast and Mold Count Testing

Yeast and mold enumeration is a key microbiological test that helps identify spoilage risks, monitor hygienic conditions, and validate the shelf stability of products prone to fungal contamination. This test is essential in acidic, low-water activity, and extended shelf-life products.

Why It’s Important

  • Spoilage Prevention: Detects organisms responsible for off-odors, gas formation, discoloration, or textural degradation.
  • Shelf-Life Validation: Ensures microbial quality over time, especially in baked goods, dairy, beverages, and condiments.
  • Hygiene Monitoring: Indicates environmental contamination and cleanliness of processing lines.
  • Fungal Safety Assessment: Supports detection of toxigenic or allergenic fungi in sensitive products.

Commonly Tested Products

  • Sauces, jams, dressings, and syrups
  • Baked goods and confectionery
  • Dairy and fermented beverages
  • Spices and dried herbs
  • RTE and vacuum-packed foods
  • Cosmetic and personal care products

Testing Methodology

  • Sample Preparation: Homogenization in sterile diluent and serial dilution where needed.
  • Selective Media: Inoculation on Dichloran Rose Bengal Chloramphenicol (DRBC) Agar or Yeast Extract Glucose Chloramphenicol (YGC) Agar.
  • Incubation Conditions: 25–28°C for 3–5 days under aerobic conditions.
  • Enumeration: Colonies are counted and reported as CFU/g or CFU/mL, with morphological notes.
  • Optional Confirmation: Microscopic observation or molecular methods if species-level ID is needed.

Accreditation & Reporting

  • Standards: Based on ISO 21527-1/2, AOAC Official Methods
  • Report Format: Digital Certificate of Analysis including media used, incubation data, CFU values, and expert interpretation
  • Accreditation: All tests performed in ISO/IEC 17025-accredited laboratories