Probiotic Count Testing
Probiotic Count Testing quantifies the number of viable, beneficial microorganisms—typically lactic acid bacteria and bifidobacteria—present in food products and dietary supplements. Accurate enumeration is essential for validating label claims, ensuring product efficacy, and maintaining consumer trust.
Purpose & Significance
- Label Claim Verification: Confirms the declared CFU (Colony Forming Units) per serving or dose, especially at end-of-shelf-life.
- Viability Assessment: Evaluates survival of probiotic strains under manufacturing, storage, and environmental conditions.
- Regulatory Compliance: Supports conformance with guidelines by FDA, EFSA, and Codex Alimentarius for functional foods and supplements.
- Stability Studies: Monitors CFU levels across shelf-life or accelerated aging protocols.
Sample Types
- Probiotic Capsules and Tablets
- Functional Beverages (e.g., kombucha, fermented juices)
- Fermented Dairy and Non-Dairy Foods
- Synbiotic Powders and Blends
- Sachets, Gummies, and Lozenges
Testing Methods
- Culture-Based Plate Count: Uses selective media such as MRS, Bifidobacteria Agar, or LBS under aerobic/anaerobic incubation. Reported as CFU/g or CFU/mL.
- Strain-Specific Enumeration: When needed, molecular tools (e.g., qPCR) or chromogenic substrates may differentiate species or strains.
- Dilution & Homogenization Protocols: Standardized procedures to ensure representative sampling, especially from capsules, powders, or viscous matrices.
- Viability Assessment Techniques: May include live/dead fluorescent staining (e.g., with PMA-qPCR) or flow cytometry in advanced testing.
Reporting & Accreditation
- Results Format: Digital Certificate of Analysis with count range, test method, and interpretive notes
- Method Standards: ISO 19344, ISO 21187, AOAC, USP <2023>/<2024>
- Accreditation: Performed under ISO/IEC 17025 accreditation
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